Endocrine Abstracts

Introduction: Despite a plethora of suggested targets and pathways, the mechanism of anti-hyperglycaemic metformin action is still unknown. The present study critically re-analysed protocols broadly applied in preclinical rodent studies.Methods: Obese male C57BL/6J mice on high fat diet were treated with metformin in the form of a single dose, daily intraperitoneal injections, admixture to drinking water, or continuous infusion via intraperitoneal minipu...

Introduction: Mountain dwellers show a lower prevalence of diabetes, which among countless other factors could be due to lower partial oxygen pressure at high altitude. The present study was to investigate in an experimental setting, if and how reduced oxygen availability affects blood glucose.Methods: Male obese mice on high fat diet were continuously maintained under a normobaric hypoxic atmosphere (10% oxygen) for three months. To exclude indirect eff...